Ng Ul To Fmol Calculator. Dilution Calculator This tool will calculate the amount of stock sol

Dilution Calculator This tool will calculate the amount of stock solution needed to achieve a desired concentration in a given volume. Mar 26, 2025 · Converting nanograms per microliter (ng/µL) to molarity (M) is a fundamental calculation in biochemistry and molecular biology. How do I get from ng/ul to fmol? Thanks! Consider the xGen™ Normalase™ Module and xGen Normalase indexing primers for enzymatic normalization of up to 1,536 libraries. 1ug/uL) (n) = (50uL) (0. A is the activity of the solid material with the activity unit expressed in units of activity per mass of material (e. ng = (fmol) x (size bp) x (660 fg/fmol) x (1 ng/106) Make up each sample to 12. I have a qubit measurement of my DNA library at 25ng/ul and the average fragment size is 1. Molarity Calculator This tool will calculate the mass needed to generate a solution based on the desired molarity, MW, and volume, or the molarity based on MW, volume, and mass. m is the mass (i. Feb 7, 2023 · Solving for the concentration of your sample in pmol/ul, you need to know the size of your DNA fragment and the concentration of DNA in ng/ul. How does the dsDNA Mass to Moles Converter calculate the conversion? DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. Then you can convert from M to uM. 7pM". This calculator is useful for diluting DNA samples. Choose the organism you DNA originates from, or choose "Custom DNA fragment". 5 ul using nuclease-free water and mix gently by pipetting. Otherwise just use the default value. , Units/mL). Mar 15, 2024 · The DNA Dilution Calculator helps scientists and researchers accurately dilute their DNA samples to a desired concentration. You should obtain 254. , Units/mg). This tool was developed to allow quick conversion from nmol or pmol to µg, as well as µg to nmol or pmol. g. I'll show my math: Where n = the volume of your [0. This calculator is useful for diluting primers and DNA oligos. Choose a DNA, RNA, or qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. The amplicon DNA concentration I have is 5. This calculator determines the dilutions needed to obtain target template concentrations in nM from samples whose length in base pairs (bp) and concentration in ng/μl is This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per μl If you know that the weight of your DNA (molar mass per bp) is different from the average weight of DNA basepair, change the value. Easy DNA molarity calculator with formula, chart, and export options. In the same way the program will recalculate answer after changing of other parameters (weight, volume, size). First, you can convert the DNA concentration from ng/ul to pmol/ul by using Avogadro's number, which is the number of atoms or molecules in one mole of a substance and is approximately 6. I have been out of the lab for sometime but I need to do a sequencing run. Whether you start with an OD₂₆₀ reading, a mass concentration, or a molar value, this tool quickly calculates the equivalents. Now, push "Calculate" in "Calculation of the molar concentration". 5ug/uL) --> n= 250uL. The weight of a molecule in Daltons equals the weight of a mole in grams. At this stage if you know the concentration in ng/uL and the MW, you don't need to use the EC for anything to determine the micromolar Jan 26, 2021 · If their assay volume is 126 µl, 1 fmol/well is equivalent to a concentration of 7. How to calculate how many microlitres I need to use to have the required 200 fmoles? This is used to convert the weight (weight concentration) into the molar quantity (molar concentration) for proteins, and vice versa. Picomoles (pmol) and Nanograms (ng) are convenient units for DNA and convert easily to larger or smaller units. Calculate DNA concentration instantly using absorbance (OD260). The calculators are numbered because sometimes the results of one calculator are used as inputs to a later one. 2kb. Also calculate molarity of solutions, perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs. What you need to do first is concentrate your stock solution. 3pM. Used to determine how […] Example calculation for converting ng/ul to nM and performing a dilution to get a desired concentration. You can divide your 1284. 8 ng/uL sample concentration by this value to get the molarity of your solution, in M. We would like to show you a description here but the site won’t allow us. Library Concentration Conversion Calculator Consider the xGen™ Normalase™ Module and xGen Normalase indexing primers for enzymatic normalization of up to 1,536 libraries. Westlab's Nucleic Acid Concentration & Molar Converter provides a seamless way to switch between all common units used for quantifying DNA and RNA. From that you can convert to ng/uL for a 1 M solution. 93 pmol/l or 1,84 pg/ml. Oct 1, 2025 · Enter the molarity, concentration, and molecular weight into the calculator to determine the missing variable. To use the ssDNA Mass to Moles Converter, you need to know the length of your ssDNA in nucleotides and either the mass of the ssDNA in nanograms (ng) or the number of moles. Whether you start with an OD₂₆₀ reading, a mass concentration, or a molar value, this tool quickly calculates the equivalents Dilute solution to a desired concentration (mass/vol). Free online tool for researchers—convert ng/µl to nM, measure purity (A260/A280), and more! Molarity Calculator The calculators on this page are independent and can be used in any order. Whether you start with an OD₂₆₀ reading, a mass concentration, or a molar value, this tool quickly calculates the equivalents To use the dsDNA Mass to Moles Converter, you need to know the length of your DNA in base pairs (bp) and either the mass of the DNA in nanograms (ng) or the number of moles. Read 3 answers by scientists to the question asked by Danielle Lewis on May 24, 2023 We would like to show you a description here but the site won’t allow us. 022 x 10²³. This calculator converts femtomoles (fmol) to nanograms (ng) based on the molecular weight of the substance. Conversions can be performed in either direction. This can be done by re-extracting it from the stock solution, and resuspending it at a lower volume. Multiply the nM indicator above for your average size (from the Bioanalyzer) by the Qubit concentration reading (ng/ul) to generate the Illumina converted concentration value for each sample. Combine the following components in a tube: 12. Apr 30, 2005 · C is the desired concentration of the final solution with the concentration unit expressed in units of activity per volume of solution (e. 75 ul Ultra II End-Prep Reaction Buffer DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. This is used to convert the weight (weight concentration) into the molar quantity (molar concentration) for nucleic acids, and vice versa. Read 3 answers by scientists to the question asked by Danielle Lewis on May 24, 2023 This protocol uses a spectrophotometer to quantify the amount (μg/mL or ng/μL) of DNA and then uses a simple equation to convert this mass concentration into a molar concentration. 1ug/uL] stock needed for your 50uL reaction: (0. Change volume for 100µl and just click somewhere outside the field (or, again, push "Calculate"): program will give "218. pmol ssDNA = [ng ssDNA/ (330 x # of bases)] (1000) ng of ssDNA = [pmol ssDNA (330 x # of bases)]/1000 As a result, we often have to convert a concentration measured in ng/μl into nM and then perform a dilution to create a solution containing the desired number of nanomoles. e. Now that we have the correct quantities of DNA fragments to add to our assembly, we can use the insert fragment lengths to calculate the mass of each insert DNA to add to the NEBuilder assembly reaction. Units of measure converter Enter a value below to perform a conversion. If you choose a custom What is the DNA Concentration Calculator? Definition: This calculator computes the concentration of a nucleic acid sample (DNA or RNA) based on its absorbance at 260 nm (A 260), using the Beer-Lambert Law. 5 ul 200 fmol amplicon DNA 1. This guide explains the process step-by-step, helping researchers and students prepare accurate solutions for experiments. Dilute solution to a desired Molarity. Jul 27, 2022 · Get these tips from IDT’s scientists on the best ways to resuspend, quantify, and calculate copy number of IDT Gene Fragments. 54 ng/ul and the size is 1200 bp. I need to load between 15-20 fmol for sequencing. Effortlessly Convert Nucleic Acid Concentrations & Assess Purity Stop juggling multiple formulas and calculators! Westlab's Nucleic Acid Concentration & Molar Converter provides a seamless way to switch between all common units used for quantifying DNA and RNA. With this information, the calculator can perform the necessary conversions. , weight) of solute that must be dissolved in volume V of solution to make the desired Access our free medical lab calculator formulas for converting relevant units. A calculator for this task is available at http://ww Convert DNA concentration (ng/µL, µg/mL) to molarity (mol/L, µM, nM) and molecules/µL. . The melatonin-AP (alkaline phosphatase ?) conjugate is used as a tracer in the assay to We would like to show you a description here but the site won’t allow us. The weight of single stranded DNA can be calculated as Molecular Weight = 330 x # of bases. The formula used for the calculation is: ng = (fmol * molecular weight) / 1000 Where: fmol is the amount in femtomoles molecular weight is the weight of one mole of the substance in grams ng is the resulting amount in nanograms Limitations Prepare 200 fmol (130ng for 1 kb amplicons) of DNA per sample in a PCR tube. Whether you are double-checking your calculations or need a reliable conversion tool, this calculator will do the trick! This calculator provides instructions on how to dilute a DNA stock solution to obtain specific DNA copy number per μL.

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